Ultra High Frequency Dielectrophoresis Manipulation to Monitor the Kinetics of Glioblastoma Cells Stemness Phenotype Acquirement
Résumé
This paper demonstrates the great potential of Ultra-High Frequency dielectrophoresis (UHF-DEP) to monitor the kinetics of glioblastoma (GBM) cells stemness phenotype transformation. Above 20 MHz, the method is able to probe the intracellular content and to be sensitive to its conductivity and permittivity values. U87-MG GBM cell line was cultured in different conditions in order to induce a differentiation gradient phenotype among the cell population. Using the presented characterization technic, it is shown than undifferentiated cells can be discriminated.
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