Nowadays organizations realize that knowledge is an important asset to achieve a competitive advantage. In the favor of that, it is necessary for organizations to manage and utilize the knowledge as much as possible through knowledge management (KM). KM concept is not only used in large companies, but also has begun to be adopted by healthcare organization in an effort to improve the quality of services. Managing knowledge is not easy, a lot of factors to consider, one of which is the culture of the organization. Organizational culture is defined as a set of practices, values, and assumptions held by members of the organization and are able to influence the behavior of the organization [12, 13]. According to Kim Cameron and Robert Quinn (2006), organizational culture can be examined using ‘Organizational Culture Assessment Instrument’ (OCAI) which has a framework, called the competing value framework (CVF). This framework consists of four culture types i.e. clan, adhocracy, market, and hierarchy. In this research, we found that healthcare organization in Indonesia have developed a dominant culture-style. It is a mix of the market and hierarchy. In addition, we also discuss the relationship of four culture types with KM, and six dimensions of organizational culture.